RESULTS & DISCUSSION
CONCLUSIONS
REFERENCES
● PI3-K – Calbiochem ( Germany )
● LY294002 – TOCRIS ( USA )
● Anti-GSK3β – New England Biolabs ( USA )
● Anti-HRP, anti-p38 – Santa Cruz Biotechnology ( USA )
▼ Phosphorylation of Akt by NGF in PC12 cells
▼ Roles of Akt in PC12 defferentiation in response to NGF
▼ Positive role of PI3-K/Akt signaling in PC12 cell differentiation
▼ Effect of NGF and inhibitors on neurite outgrowth of PC12 cells
We found that neurite outgrowth in the PC12 cells was stimulated by NGF and that ML-9, an Akt inhibitor, prevented this outgrowth.
Moreover NGF-induced neurite outgrowth was greatly enhanced in cells overexpressing a constitutive form of Akt, CA-Akt,
and dramatically inhibited in cells expressing a dominant negative Akt.

분야