논문 > 자연계열

[논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery

 1  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-1
 2  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-2
 3  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-3
 4  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-4
 5  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-5
 6  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-6
 7  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-7
 8  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-8
 9  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-9
 10  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-10
 11  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-11
 12  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-12
 13  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-13
 14  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-14
 15  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-15
 16  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-16
 17  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-17
 18  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-18
 19  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-19
 20  [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery-20
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    [논문발표 PPT] Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery에 대한 자료입니다.
    본문내용
    1. Organ bath study
    Animal : New Zealand white rabbits coronary arterial (4-5mm in length)
    Endothelium of the arterial ring was remove
    0Ca2+-1mM EGTA sol. : KH sol. and addition of 1mM EGTA

    2. Single-cell isolation and Ca2+ imaging
    Stips loaded with 1uM fura-2/AM in Tyrode’s solution for 30min at room temperature
    [R(F340/F380)], 510nm CCD camera
    Dissociation constant of fluorescence indicator for Ca2+

    3. Permeabilization with ß-escin and force recording
    Permeabilized strips : 200um wide and 2-3mm long
    Permeabilization : Incubated at room temperature in ß-escin(30uM) in relaxing sol. (4.5mM MgATP, 1mM EGTA etc.) for 25min


    Our results suggest that constriction of arteries in response to the bioactive lipid S1P or SPC occurs by distinct signalling pathways. Activation of the RhoA/RhoA-associated kinase pathway and subsequent phosphorylation of MYPT1 play a key role in SPC-induced coronary contraction, whereas elevation of [Ca2+]i is crucial for S1P-induced coronary constriction.
    참고문헌
    Cardiovasc. Res. 2009 Feb. 82:324-332
    하고 싶은 말
    대학원 석사과정 때 위 논문을 읽고 발표한 PPT입니다.
    PPT에 발표 스크립트도 포함되어 있습니다.
    논문읽고 발표수업 하실 때 참고하시면 많은 도움 되실겁니다.
    #논문발표# 의학논문# 자연과학논문# 졸업논문발표# 졸업논문